
Plant tissue culture initiation and subculture kit
Kit for demonstrating aseptic handling of explants, establishing cultures and subsequent subculturing.
The kit demonstrates sterile handling of explants, the initial introduction of plant material into culture and subsequent subculturing. Students learn the principles of asepsis in plant biotechnology and practise treating explants and instruments, placing explants on nutrient media and transferring cultures. These procedures provide basic experience in micropropagation.
- 1.Sodium hypochlorite (10 g) - 1 bottle
- 2.Safety glasses - 1 pc.
- 3.Scissors (18 cm) - 1 pc.
- 4.Tweezers (23 cm) - 1 pc.
- 5.Petri dish (75 mm) - 2 pcs.
- 6.Scalpel assembly (handle No. 4 + blade No. 23) - 1 set.
- 7.Battery-powered timer - 1 pc.
- 8.Beaker (250 ml) - 1 pc.
- 9.Bottle for reagent (500 ml) - 1 pc.
- 10.Qualitative filter paper (7 cm / 100 pcs) - 1 box
- 11.Ethanol 75% (250 ml) - 1 bottle
- 12.Glass rod (20 cm) - 1 pc.
- Selection and preparation of explants (selection of young healthy plant parts).
- Sterilization of explants: sodium hypochlorite and 75% ethanol.
- Establishing a culture: placing explants on prepared MS medium.
- Passaging (subculture): transfer of a growing culture to fresh medium.
- Monitoring the development of callus and the formation of new organs.
High school
advanced level
Plant micropropagation. Aseptic handling of explants (elective classes in agrobiotechnology)
- — Selection and preparation of explants (young healthy parts of the plant)
- — Sterilization of explants with sodium hypochlorite and 75% ethanol
- — Introduction of explants into culture on prepared MS medium
- — Subculturing; monitoring callus development and organ formation
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