Culture medium preparation kit for plant tissue culture
Kit for preparing culture media, adjusting their pH and dispensing them while learning the basics of plant tissue culture.
The kit introduces plant tissue culture through preparing culture media, adjusting pH and dispensing the media into containers. Students study the composition and purpose of MS medium and practise precise pH adjustment and sterilisation by autoclaving. The activities also introduce sterility requirements and the basic equipment of a biotechnology laboratory.
- 1.Double-sided spatula-spoon (18 cm) - 1 pc.
- 2.Weighing paper (50 × 50 mm) - 1 pack (500 pcs)
- 3.Reagent bottle (125 ml) - 1 pc.
- 4.Pen-type pH meter (17 × 155 × 31 mm) - 1 pc.
- 5.Glass rod (20 cm) - 1 pc.
- 6.Cultivation vessel (40 ml) – 10 pcs.
- 7.Granulated sugar (carbon source) (20 g) - 1 pack
- 8.Tripod (10 × 15 cm) – 10 pcs.
- 9.Alcohol lamp (150 ml) - 1 pc.
- 10.Ethanol 75% (250 ml) - 1 bottle
- 11.Asbestos mesh (12.5 × 12.5 cm) - 1 piece
- 12.HCl solution (20 ml) - 1 bottle
- 13.NaOH solution (20 ml) - 1 bottle
- 14.Beaker (250 ml) - 1 pc.
- 15.Gelling powder (analogue of agar) (50 g) - 1 pack
- Preparation of MS (Murashige–Skoog) nutrient medium, a classic medium for plant tissue culture.
- Adjusting the pH of the medium with HCl and NaOH solutions to the target value of 5.8.
- Sterilization of the medium by autoclaving.
- Filling and solidification of the medium in sterile cultivation vessels.
High school
advanced level
Biotechnology. Culture of plant cells and tissues (elective classes in agrobiotechnology)
- — Preparation of Murashige–Skoog (MS) culture medium
- — Adjusting the pH of the medium with HCl and NaOH solutions to a value of 5.8
- — Sterilization of media by autoclaving
- — Filling and solidification of the medium in sterile cultivation vessels
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